SeerPharma Pty Ltd :: DISCover web-based training :: QC 9: Microbiology Laboratory Techniques

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QC 9: Microbiology Laboratory Techniques

Description: This module describes some fundamental techniques and procedures that are used regularly in microbiology laboratories.

Price: $239 (AUD)

(Australian customers, please add GST.)

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COURSE OUTLINE

INTRODUCTION
1. Course Overview
2. Overview
3. Assessment

LAMINAR FLOW AND BIOHAZARD CABINETS
4. Introduction
5. Airflow patterns I
6. Airflow patterns II
7. Transferring materials
8. Review

MEDIA PREPARATION AND QC
9. Introduction
10. Importance of media
11. Media preparation notes
12. QC tests
13. Storage conditions
14. Importance of traceability
15. Review

READING EXPOSURE PLATES
16. Introduction
17. Environmental monitoring
18. Microbiological plating
19. Membrane filtration
20. Direct plate techniques
21. Exposure plate reliability
22. Reading and recording
23. Review

METHOD VALIDATION FOR MICROBIOLOGY
24. Introduction
25. Test methods
26. Typical validation tests
27. Examples

HANDING AND MAINTAINING CULTURES
28. Introduction
29. Why maintain cultures?
30. Sources of cultures
31. Cultures as references
32. Precautions
33. Subculturing requirements
34. Review

THE STERILITY TEST
35. Introduction
36. Purpose & principles
37. Unsatisfactory test results
38. Satisfactory test results
39. Sterility test environment
40. Negative
41. Review

CONCLUSION
42. Summary

COURSE OBJECTIVES

Students completing this course should be able to:

Work effectively in a cleanroom cabinet
Describe how media is prepared and quality controlled
Read plates and report results
State the requirements for microbiology method validations
Handle and maintain cultures according to laboratory procedures
State the special requirements and limitations of the sterility test

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