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Methods in Molecular Biology

Description: This course is an introduction to molecular biology, with a focus on the techniques used to isolate, identify, modify and analyze three key molecules: DNA, RNA and proteins. The course describes the principle concepts and uses of each technique, using a case study to illustrate the relevant points. The course does not cover the protocols for these techniques.

COURSE OUTLINE

INTRODUCTION
1. Course Information
2. Interface Tour
3. Course Overview

FUNDAMENTALS
4. Objectives
5. Central Dogma
6. DNA/RNA Structure
7. Translation
8. Protein Structure
9. Progress Check

CLONING
10. Objectives
11. DNA Manipulation
12. DNA Cloning and Gene Transfer
13. DNA Sequencing
14. Recombinant Proteins
15. Case Study: Part 1
16. Progress Check

POLYMERASE CHAIN REACTION
17. Objectives
18. PCR
19. Real time PCR
20. RT-PCR
21. Case study: Part 2
22. Progress Check

SEPARATION TECHNIQUES
23. Objectives
24. DNA and RNA Electrophoresis
25. SDS-PAGE
26. Native Gels
27. IEF
28. 2D Gel Electrophoresis
29. Chromatography
30. Case study: Part 3
31. Progress Check

BLOTTING TECHNIQUES
32. Objectives
33. Southern and Northern
34. Western
35. Case study: Part 4
36. Progress Check

COURSE OBJECTIVES

Students completing this course should be able to:

Describe the basic structure and function of DNA, RNA and Proteins
Explain the "Central Dogma"
Understand the steps in the translation of RNA to protein
Explain how DNA is manipulated
Describe how a fragment of DNA is cloned
List three methods of introducing DNA into cells
Explain how recombinant proteins are generated
Explain how DNA is sequenced
List the reagents needed to perform PCR
Explain the concept of PCR
Explain how RT-PCR is used for gene expression analyses
List and explain the four common types of "real time PCR
Explain how RNA and DNA are separated using electrophoresis
Explain how proteins are separated using electrophoresis
Explain how large mixtures of proteins can be analyzed
Specify the main steps of chromatography
List and describe the five types of chromatography
Explain how Southern, northern and western blotting are performed
Specify the uses of each technique

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