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Microarrays

Description: The microarrays course describes the various chip fabrication techniques, image and data analysis, and the uses of microarrays for re-sequencing, expression studies and genotyping.

COURSE OUTLINE

INTRODUCTION
1. Course Description
2. Interface Tour
3. Historical Perspective
4. Course Overview

PROBES
5. Objectives
6. Types of Probes
7. Progress Check

ARRAY FABRICATION
8. Objectives
9. Photolithography
10. In Situ Oligonucleotides
11. Oligonucleotide Deposition
12. cDNA Preparation
13. DNA Arrays
14. Array Surfaces
15. Progress Check

TARGETS
16. Objectives
17. Types and Labeling
18. RNA Amplification
19. Progress Check

ASSAY
20. Objectives
21. Hybridization Conditions
22. Progress Check

READOUT
23. Objectives
24. Why Fluorescence?
25. Scanning Technology
26. Progress Check

IMAGE ANALYSIS
27. Objectives
28. Quantification
29. Normalization
30. Informatics
31. Cluster Analysis
32. Progress Check

USES/EXAMPLES
33. Objectives
34. Sequencing
35. Genotyping-HIV
36. Expression Analyses
37. Liquid Arrays-MPSS
38. Progress Check

CONCLUSION
39. Summary and Future Trends

COURSE OBJECTIVES

Students completing this course should be able to:

List the three common types of polymorphisms
Define what a SNP is and how it originates
Describe the three types of methods used to identify and detect SNPs
Specify the resources that are available for SNP analyses
Calculate the normalized rates of SNP occurrence
List two examples of SNP genotyping methods
Define the requirements of genotyping assays
Explain the use of SNPs as genetic markers
Describe the use of SNPs for association studies

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